ywlE [2019-02-19 17:28:25]

protein arginine phosphatase

locus: BSU36930

pI: 6.68

mw: 16.64 kDa

protein length: 150 aa

gene length: 453 bp

function: dephosphorylation of McsB

product: protein arginine phosphatase

essential: no

ec: 3.1.3.48

synonyms: ipc-31d

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.4|Protein phosphatases]

Gene

Coordinates: 3,791,805 3,792,257

The protein

Catalyzed reaction/ biological activity

• Protein arginine phosphate + H₂O = protein arginine + phosphate [Pubmed|22517742]

Paralogous protein(s)

• [protein|2CEFD8D9692D062CC09E3DEDA1A4015C76BC4167|YfkJ]

Effectors of protein activity

• activity is inhibited by oxidative stress [Pubmed|27524296]

Structure

• [PDB|1ZGG]

[SW|Localization]

• weak oligomerization, results in inactivation of YwlE [Pubmed|19678837]

Expression and Regulation

Operons

• genes: [gene|19829F6D993188BE854F0F61CB93777AD04CF754|ywlD]-[gene|1BAD28287257F82F907706AFD2E8FF5F3BD1B57B|ywlE]-[gene|91827FEC70F5EEB8B072D06ABA05C19FC54D5F71|ywlF]-[gene|41407DE15D91959DA92799623F1364811C170F35|ywlG]
  description: [Pubmed|22383849]

• genes: [gene|1BAD28287257F82F907706AFD2E8FF5F3BD1B57B|ywlE]-[gene|91827FEC70F5EEB8B072D06ABA05C19FC54D5F71|ywlF]-[gene|41407DE15D91959DA92799623F1364811C170F35|ywlG]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A186 (ywlE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/186 NBRP B. subtilis, Japan]
• ''ywlE::aphA3'' available from [SW|Ulf Gerth]'s lab
• ''ywlE::tet'' available from [SW|Ulf Gerth]'s lab
• ''ywlE::spec'' available from [SW|Ulf Gerth]'s lab
• GP1458 (''ywlE''::''aphA3''), available in [SW|Jörg Stülke]'s lab
• GP1459 (''ywlE''::''tet''), available in [SW|Jörg Stülke]'s and [SW|Fabian Commichau]'s labs [Pubmed|25610436]
• BKE36930 ([gene|1BAD28287257F82F907706AFD2E8FF5F3BD1B57B|ywlE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCAGTCACCCCTTATT, downstream forward: _UP4_TAAGTTGTCAGAAAATCTGC
• BKK36930 ([gene|1BAD28287257F82F907706AFD2E8FF5F3BD1B57B|ywlE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCAGTCACCCCTTATT, downstream forward: _UP4_TAAGTTGTCAGAAAATCTGC

Expression vectors

• for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [SW|Ulf Gerth]'s lab
• expression of native ''ywlE'' in ''B. subtilis'': pBP183 (in [SW|pBQ200]), available in [SW|Fabian Commichau]'s lab

Antibody

• available in [SW|Ulf Gerth]'s lab

labs

• [SW|Ulf Gerth], Greifswald, Germany
• [SW|Fabian Commichau] Göttingen, Germany

References

Reviews

• 27541193,28748186

Original Publications

• 16452434,15866923,15995210,19678837,21622759,20852588,22517742,23770242,23838530,24263382,24825175,25610436,27524296